DC-2 BIOCHEMISTRY AND HUMAN NUTRITION


1. One pg is equal to





2. Imidazole ring is present in amino acid named





3. Given below is not a positively charged amino acid





4. An enzyme that catalyses the reaction A B changes the





5. Digestive enzyme Trypsin and Chymotrypsin cleaves a protein/ polypeptide specifically





6. The enzyme which can add water to a carbon-carbon double bond or remove water to create a double bond without breaking the bond is





7. Pepsin is an example for the class of enzymes





8. The co-enzyme not involved in Hydrogen transfer





9. Which of the following properties is not characteristic of enzymes?





10. Enzymes accelerate reaction rates by:





11. Which of the following statements about enzyme reactions is false?





12. What is an active site?





13. Why is the Lineweaver-Burk plot important in enzyme kinetics?





14. Fischer’s ‘lock and key’ model of the enzyme action implies that





15. In Induced Fit model of enzyme substrate binding





16. Many enzymes that have NAD+ or NADH as a substrate exhibit





17. In double displacement mechanism of enzyme action





18. In bi-substrate ordered enzymatic reactions





19. The pH optima of most of the enzymes is





20. Factors affecting enzyme activity





21.

The isoenzymes LDH5 is elevated in







22.

Coenzymes combine with







23.

Isoenzymes are







24.

The kinetic effect of purely competitive inhibitor of an enzyme







25.

In non competitive enzyme activity inhibition, inhibitor







26. Malonate is an inhibitor of Succinate dehydrogenase





27. In Un-competitive inhibition Inhibitor binds with





28.

In competitive enzyme activity inhibition







29. If a plot of v vs. [S] is sigmoidal, it most likely means that







30.

What are allosteric regulators?







31.

A characteristic of the active site of lysozyme that is a common feature of many enzymes is







32.

An inducer is absent in the type of enzyme:







33. Activation or inactivation of certain key regulatory enzymes is accomplished by covalent modification of the amino acid:





34.

The specific activity of an enzyme is







35. The technique where large size substrates have difficulty in approaching the catalytic sites of immobilized enzymes





36. The technique for immobilization of enzymes where chances of leakage from support is minimum.





37. Generally on immobilization, the specific activity of enzyme





38. Most commonly used amino acid residue of enzymes involved in covalent binding and cross-linking during immobilization





39. Enzymes, which are produced in inactive form in the living cells, are called





40. Chymotrypsinogen and proelastases are activated by the action of proteolytic enzyme





41. Pepsinogen secreated in stomach is activated by





42. The molecular machine of ribozomes that translates RNA into proteins has catalytic activity known as





43. Proteolytic activity of secreted digestive enzymes is regulated by





44.

In enzyme assays, all the following are kept constant except







45.

Vmax of an enzyme may be affected by







46. One Unit of enzyme is defined as





47. Instrument not used to measure Enzyme activity is





48.

Alkaline phosphatase is present in







49. In EM pathway -2-phosphoglycerate is converted to





50. Under anaerobic conditions the glycolysis one mole of glucose yields __ moles of ATP.





51. The following is an enzyme required for glycolysis:





52. Which of the following is a substrate for aldolase activity in Glycolytic pathway?





53. The glycolysis is regulated by





54.

The conversion of alanine to glucose is termed







55.

Which of the following reactions is unique to gluconeogenesis?







56. In presence of the following cofactor, pyruvate carboxylase converts pyruvate to oxaloacetate:





57.

How many ATP molecules will be required for conversion of 2-molecules of Lactic acid to Glucose?







58.

Oxaloacetate produced in mitochondria by pyruvate carboxylase in gluconeogenesis







59. How many ATPs are produced in the conversion of phosphoenol pyruvate to citrate?





60. Which of the following statements regarding T.C.A cycle is true?





61. The conversion of pyruvate to acetyl CoA and CO2





62. An allosteric enzyme responsible for controlling the rate of T.C.A cycle is





63.

The enzymes of the citric acid cycle







64.

An essential for converting Glucose to Glycogen in Liver is







65.

A regulator of the enzyme Glycogen synthase is







66. Name the following pathways: Releases glucose from glycogen






67.

Glucose is released by glycogen phosphorylase as







68.

Glycogen synthase







69.

What is the name of the pathway that degrades fatty acids (from ingested triacylglycerols) to acetyl-S-CoA?








70.

Essential fatty acid







71.

During each cycle of ?-oxidation







72.

Activation of fatty acids requires all the following except







73. Carnitine is required for the transport of





74.

High iodine value of a lipid indicates







75.

Intermediate in the denovo synthesis of triacyl glycerols include all the following except







76. A pathway that requires NADPH as a cofactor is





77.

Chain elongation of fatty acids occurring in mammalian liver takes place in which of the following subcellular fractions of the cell?







78.

Acyl carrier protein is involved in the synthesis of







79. All of the following statements about the enzymic complex that carries out the synthesis of ATP during oxidative phosphorylation are correct except





80. Electron transport and phosphorylation can be uncoupled by compounds that increase the permeability of the inner mitochondrial membrane to





81.

If the more negative standard reduction potential of a redox pair, the greater the tendency to







82.

The more positive the E0, the greater the tendency of the oxidant member of that pair to







83.

The standard free energy of hydrolysis of terminal phosphate group of ATP is







84.

Sulphur containing amino acid is







85.

The biosynthesis of Urea begins in the Liver:







86. The end product of amino acid nitrogen metabolism in uricotelic organisms (reptiles and birds) is





87.

The transaminase activity needs the coenzyme:







88.

The only amino acid that undergoes oxidative deamination at an appreciable rate







89. _________ is the body’s primary source of energy





90.

Dietary fiber is an indigestible _________ that serves separately as a body regulatory agent.







91.

Which of the following is called a silent nutrient?







92.

Which of following is an essential amino acid?







93. Body protein has a source of energy of





94.

Breaking down of larger substances into smaller units is called







95.

The amount of nutrients a food contains in relationship to number of calories it contains







96.

The below mentioned statements regarding Recommended Dietary Allowances (RDA) is false







97.

RDA values are not given for







98.

The value of protein RDA is







99. Heat of reaction at a constant volume is measured in the apparatus





100. Protein content in food products is mostly measured by





101. In Kjeldahl method protein is estimated by estimation of





102. Phenol sulphuric acid method is used to estimated _________ in food





103. Ether extraction for lipid estimation in food products is a





104.

One manifestation of vitamin A deficiency is







105.

The vitamin which would most likely become deficient in an individual who develop a completely carnivorous life style is







106.

Vitamin B1 coenzyme (TPP) is involved in







107.

Scurvy is caused due to the deficiency of







108.

Vitamin essential for transamination is







109.

Hormones







110.

Epinephrine stimulates glycogenolysis in







111. Which one of the following hormones is derived most completely from tyrosine?





112.

A hormone used for detection of pregnancy is







113.

All mammalian steroid hormones are formed from







114.

Emulsifying agent produced by the liver and stored in the gall bladder aids fat digestion and absorption.








115. Most of the hydrolysis of triglycerides occurs in the





116.

Digestion in the small intestine is where pancreatic amylase converts the carbohydrate starch to







117.

The zymogen pepsinogen, is converted to pepsin in the acid medium (autoactivation) or by active pepsin (autocatalysis)







118.

The proteolytic pancreatic enzymes include







119.

Galactose is phosphorylated by galactokinase to form







120. Lactose intolerance occur due to the deficiency of enzyme





121.

Galactosemia occurs due to impairment in metabolism of







122.

The patients of phenyketonuria should restrict _________ amino acid in their diet.







123.

Milk hypersensitivity is a







124.

Majority portion of Milk fat is







125.

The fatty acids used to synthesize milk fat (triglycerides) come from







126.

Major protein of milk whey







127.

Milk proteins are synthesized in







128.

Enzyme responsible for lactogenesis appear







129. PEM stands for





130. Iodine efficiency causes





131. National Institute of Nutrition is situated at





132. Goitre Control Programme was initiated in





133. Mid-Day Meal Programme was first launched in





134. Which one is not an Antioxidant





135. WHO stands for





136. Mg is a Chelating Agent found in





137. Solanine a natural toxicants that interferes with transmitting nerve impulses found in





138. Citral a natural toxicant found in orange peel is antagonist to





139. Recommended Daily requirement of Vitamin C is





140. Vitamin C deficiency causes





141. Vitamin c is soluble in





142. Vitamin C activates an enzyme Prolyl hydroxylase that help in the formation of a collagen constituent known as





143. Average concentration of Cholesterol in blood is





144. Structurally, why is starch a better molecule for energy storage than cellulose?






145. Starch and glycogen are different in that only one of them:





146. Common fatty acids have





147. Which of the following is TRUE of sphingolipids





148. Essential fatty acids





149. The DNA polymer (5')GTGATCAAGC(3') is complementary to and could form a double-stranded structure with





150. When messenger RNA code is translated, what new product forms?





151. What term describes the phosphate, carbon, and nitrogenous base groups that make up DNA?





152. The visible range for spectrophotometer lies between





153. The ratio of 260 nm/280 nm of a pure DNA solution lies between





154. Which method is not used for protein estimation?